Ordoñez AA1, Ordoñez RM, Zampini IC, Isla MI.
The aims of the present study were to determine the antibacterial and
antifungal activity as well as mutagenicity of Sechium edule fluid
extract and to obtain a pharmaceutical formulation with them. The
extract exhibited antimicrobial activity against Escherichia coli,
Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Serratia
marcescens, Morganella morganii, Acinetobacter baumannii, Pseudomonas
aeruginosa, Stenotrophomonas maltophilia, Candida spp. and Aspergillus
spp. isolated from clinical samples from two hospitals of Tucuman,
Argentina. Non-toxicity and mutagenicity on both Salmonella typhimurium
TA98 and TA 100 strains until 100 microg/plate were observed. A hydrogel
with carbopol acrylic acid polymer containing S. edule fluid extract as
antibacterial, antimycotic and antioxidant agent was obtained.
Microbiological, physical and functional stability of pharmaceutical
formulation conserved at room temperature for 1 year were determined.
Addition of antioxidant preservatives to store the pharmaceutical
formulation was not necessary. The semisolid system showed antimicrobial
activity against all gram positive and gram negative bacteria and fungi
assayed. The minimal inhibitory concentration (MIC) values ranged from
20 to 800 microg/mL. Its activity was compared with a pharmaceutical
formulation containing commercial antibiotic and antifungal. A
pseudoplastic behavior and positive thixotropy were observed. Our
current finding shows an antimicrobial activity of hydrogel containing
S. edule extract on a large range of gram negative and gram positive
multi-resistant bacteria and fungi. This topical formulation may be used
as antimycotic and as antibacterial in cutaneous infections.